Guide to gDNA Extraction

DNA and RNA extraction and purification are vital tools for research and clinical applications. Often, researchers are looking for a microbe’s genome within the host’s genomic DNA which may be a billion-fold. The focus is on quicker and high-precision nucleic acid extraction products.

Fortunately, technology improvements have made highly refined DNA and RNA purification possible. For example, MagBio Genomics’ HighPrep™ Blood & Tissue DNA Kit is a magnetic bead-based kit that allows high-quality genomic DNA extraction from a variety of fresh or frozen blood and tissue samples.

How does it work?

The HighPrep™ blood & tissue DNA Kit includes AS Buffer, TS Buffer, HSW Buffer, MB Elution Buffer, Pro K Solution and MAG-S1 particles.

The extraction is a simple 3 step procedure –

Step 1: Sample lysis; DNA binds to the MAG-S1 magnetic beads.

Step 2: Separation of the bound genomic DNA from the solution (using a magnetic separation device) and washing.

Step 3: The final step is the elution of high-quality genomic DNA from the magnetic beads.

The result is high quantity and quality DNA yields that can be utilized directly in various genomic downstream applications.

This blood and tissue DNA Kit can extract high-quality genomic DNA from 50-250 µL of fresh or frozen –

• whole blood
• buffy coat
• lysate of tissues
• mouse tails
• cultured cells
• saliva
• buccal swabs

Applications of HighPrep™ Blood & Tissue DNA Kit

The kit allows blood and tissue gDNA extraction for:

• PCR, Real-time PCR
• Southern Blotting
• Cloning, Genotyping
• Sequencing

Benefits of HighPrep™ Blood & Tissue DNA Kit

• High gDNA yield and pure samples from blood and tissue samples
• No organic extraction or alcohol precipitation
• Complete removal of contaminants and inhibitors
• Can be used for a few samples as well as in high throughput settings on automated workflow

When compared with other commercially available kits, HighPrep™ blood & tissue DNA Kit shows –

• Higher DNA integrity
• Better DNA yields

If you are looking for high-quality magnetic beads for PCR clean up and kit for normalization of DNA concentration, call one of the most trusted RNA extraction kit manufacturers in the USA, MagBio Genomics at (301) 302-0144.