Tips for Successful Genomic DNA Extraction

Genomic DNA extraction allows scientists to study genes, carry out entire genome sequencing, modify parts of DNA, and more. In order to ensure the success of these downstream applications, the highest quality DNA is needed. Let’s look at some great tips for genomic DNA purification.

Sample quantity

Each sample can have a different amount of DNA. For example, nucleated blood carries more DNA than a muscle tissue sample. It is bests to follow the sample loading guidelines to avoid adverse effects on lysis efficiency and kit performance.

Sample type

Lysis is one of the most important steps in any genomic DNA extraction. It needs to be completed properly. Incorrect lysis will reduce DNA yield as well as purity. The best lysis conditions depend upon the sample type.

·         Biological fluids and cells

Biological fluids, such as saliva and blood, and cells require a quick proteinase K digestion at 55°C. The sample should be completely homogeneous after digestion.

·         Tissues

Tissues require more intense lysis. The tissue should be cut up to increase the surface area and to allow more space for lysis by proteinase K. This will accelerate digestion and incubation time.

·         Bacteria and Fungi

Cell walls in bacteria and fungi make lysis difficult. Enzymatic, chemical, and physical methods can help to ensure complete lysis.

·         Plants and Seeds

Plant tissues have thick cell walls, which are resistant to chemical lysis. To ensure complete cell lysis, plant tissue must be cut into or ground before the physical method of lysis.

Handling also plays a critical role in genomic DNA extraction. So, it is best to work at a pace you are comfortable with.

Extracting DNA can be daunting, but all-inclusive products, like the HighPrep™ Viral DNA/RNA Kit by MagBio Genomics, can simplify the extraction procedure.

If you are looking for a kit for the normalization of DNA concentration, or a Sanger sequencing reaction clean-up kit, call one of the most trusted DNA/RNA extraction kit manufacturers in the USA, MagBio Genomics at (301) 302-0144.